MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY

E 729                                       (Pages: 3)                                    Reg. No …..…….

                                                                            Name ……...……

B.Sc. DEGREE (CBCSS) EXAMINATION, NOVEMBER 2010
Third Semester
B.Sc. Botany and Biotechnology (Double Main)
Core Course—MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY
Time : Three Hours                                                                                          Maximum Weight: 25
Part A
Answer all questions.
Weight I each for a bunch of four.

Bunch I

1. The image will be darker than the background around it in:
            (a) Light microscopy                (b) Fluorescent microscopy.
            (c) Dark field microscopy.       (d) TEM.
2. Peptidoglycan is present in:
            (a) Gram +ve bacteria.             (b) Gram —ye bacteria.
            (c) Virus.         (d) Fungi.
3. Sterilization by a course of three periods of bailing at 100°C for 30 minutes at daily intervals is called:
            (a) Tyndallization.       (b) Pasteurization.
            (c) Filteration. (d) Radiation.
4. Pigment bearing membranous structure in photosynthetic bacteria is called:
            (a) Chioroplast.                        (b) Chromoplast.
            (c) Chromatophores.    (d) Leucoplast.

Bunch II

5. The process of ATP synthesis at the expense of protonmotive force is called:
            (a) Exosmosis.                         (b) Endosmosis.
            (c) Chemiosmosis.       (d) Diffusion.
6. Hot air oven is used for:
(a) Dry heat sterilization.         (b) Moist heat sterilization.
(c) Chemical sterilization.        (d) Radiation sterilization.

Turn over

2

7. Movement of bacteria toward or away from chemical stimuli is called:
            (a) Chemotaxis                        (b) Photo taxis.
            (c) Hydrotaxis.             (d) Nastic movement.
8. The bacterium that carries the prophage is said to be:
            (a) Lysogenic.  (b) Pathogenic.
            (c) Virulent.     (d) Prophage.

Bunch III

9. Humulin is produced by:
            (a) Bacteria.        (b) Fungi.
            (c) Algae.         (d) Virus.
10. Sterilization by steam under pressure is carried out at temperature between:
            (a) 108° C and 147° C.cZ                    (b) 160° C and 168° C.
            (c) 98° C and 108° C.               (d) 1600 C and 190° C.
11. Which of the following enzyme is thought to be useful for detergent manufacture?
            (a) Amylase.    (b) Protease. .../
            (c) Rennet.       (d) Lactase.
12. Mac Conkeys medium is an example of:
            (a) Differential medium.          (b) Indicator medium.
            (c) Selective medium.  (d) Enriched medium.

Bunch IV

13. Five kingdom classification was proposed by:
            (a) R.H. Wbittaker.      (b) T.H. Morgan.
            (c) Jacques Monod.     (d) Haeckel.
14. One of the following is a biopesticide:
            (a) Bacillus thuringjensjs                      (b) Bacillus megatherjum.
            (c) Rhizobjum.                         (d) Azotobacter.
15. Which of the radiation is ionisable?
            (a) Infra-red.    (b) Ultra-violet.
            (c) Gamma.      (d) Visible.
16. The fungus used as a weedicjde:
            (a) Phytoththora palmivora.     (b) Rhizopus.
            (c) Pythium.                                     (d) Agaricus.

(4 x 1= 4)

Part B
Answer any five questions.
Weight 4 each.

17.  What is glycocalyx?
18. What is dark field microscopy?
19. What is the function of cotton plug in Microbiology?
20. What is infusion broth?
21. What is spheroplast?
2 2. Explain the structure and function of flagella.
23. W’natareprions?
24.  Explain lysogeny.

(5 x 1 5)

Part C
Answer any four questions.
Weight 2 each.

25. Write notes on biofuels?
26. Explain microencapsulation techniques.
2’7. What are the major bacterial antibiotics?
28. Describe Plackett-Burman design.
29. Explain the structure of viruses.
3O. What are antiviral drugs ? Give examples.

(4 x 2 = 8)

PartD
Answer any two questions.
Weight 4 each.

31. Explain the structure and types of bioreactors.

32. Describe the economic importance of microbes.
33. What are the different methods to classify bacteria?

(2 x 4= 8)